Amazing Spider-Man and Spider-Man 2099 will return in Spider-Man: Edge of Time, the follow-up to Spider-Man: Shattered Dimensions, set to arrive on Xbox 360 and PlayStation 3 in autumn 2011.
Both Amazing Spider-Man and his futuristic equivalent appeared in Shattered Dimensions, along with Spider-Man Noire and Ultimate Spider-Man.
Shattered Dimensions brought together Spider-Men from four different realities, while Edge of Time focuses on two - and once again both are threatened by a common enemy.
Prolific comic book writer and 2099 co-creator Peter David wrote the storyline for Edge of Time, having already been involved in the 2009 Xbox Live Arcade hit Shadow Complex and several Ben 10 episodes.
Beenox, the same studio behind Shattered Illusions, is working on Edge of Time.
The studio's publisher, Activision, has also given the go-ahead for a new Transformers game from High Moon Studios.
Transformers: Dark of The Moon, which will act as late June prequel to July's movie of the same name, builds on the success of Transformers: War for Cybertron.
It's a good year for games based on cartoons, comics, and graphic novels: DC Universe Online came out in January, with Marvel vs Capcom 3 following in February and Lego Star Wars III: The Clone Wars in March.
The Smurfs video game will tie-in with an August motion picture, while for older players Batman: Arkham City is due in October.