The programme segmented old footage of Miss Dando with tributes from her fiancee Alan Farthing and co-presenter Nick Ross. Miss Dando told viewers: "We rely on you to spot the clues and recognise the details that hopefully will lead to criminals being caught."
The programme centred on witnesses who saw the mobile-phone carrying suspect near her home and police efforts to trace an aggressively driven blue Range Rover in the area around her Fulham home on the morning of her murder.
Detective Chief Inspector Hamish Campbell, who heads the murder inquiry, reiterated the theory that two men may be implicated in the murder. One acting as look-out for the killer.
Last night's Crimewatch was the first edition of the programme since Miss Dando was gunned down outside her home on 26 April.
At least eight million viewers were estimated to have tuned in. Hundreds of calls were received including some from Range Rover owners and a new witness who was on a bus nearby at the time of the murder.