"I was notified by the state attorney's office that based on the DNA testing, my client has been excluded as a suspect," defence lawyer Diana Tennis told the Orlando Sentinel.
Kenneth David Taylor could have charges dropped against him immediately.
Mr Taylor, 39, was accused of robbing a British couple in their motel room, forcing them to have sex with one another and then raping the woman.
He was arrested three days after the attack on 13 July after one of the victims identified him from a photograph as the attacker. Mr Taylor said he was in Atlanta working at the time. He was one of two men arrested in connection with a series of 10 attacks in central Florida.
The other man, Eugene Holston, 34, was charged last week with another of the attacks. Osceola County sheriff's detectives said DNA test results showed a positive match for Holston and that both victims in that attack had identified him from photographs.
Mr Taylor was released from jail on bail two weeks ago but has had to wear an electronic monitoring bracelet while awaiting the results of the DNA test.Reuse content