Coleen McLoughlin confirmed today that her brother was arrested over the brawl which saw football star Steven Gerrard charged with assault.
The England and Liverpool midfielder will appear before magistrates later this month to face allegations of affray and assault after a fight at the Lounge Inn in Southport, Merseyside.
Yesterday, a 19-year-old from Huyton, Liverpool, was arrested in connection with the brawl - in which part-time DJ Marcus McGee allegedly lost a tooth and suffered a cut face.
Today, a spokesman for Coleen, 22, who is married to Manchester United ace Wayne Rooney, confirmed that Anthony McLoughlin was taken into custody.
He said: "All we can say is to confirm he was arrested and confirm he was released without charge.
"There will be no other comment from Coleen, Wayne or Coleen's family."
McLoughlin's father, Tony, 49, told the Daily Mirror his son had "not done anything", adding: "Everything is all right, he is out, he is all right."
Merseyside Police said McLoughlin was released pending further inquiries.
Gerrard was celebrating the Reds' win over Newcastle when the trouble began.
The international was arrested and interviewed by detectives who later charged him.
In the hours after the fracas, blood and broken glass were visible on the floor of the club.
Gerrard, of Formby, Merseyside, will appear at North Sefton Magistrates' Court on January 23 with John Doran, 29, and Ian Gerard Smith, 19, both of Huyton.
They also face counts of assault and affray.