Charles William Acquaah, 22, of Greenside Road, Croydon, south London, was tonight charged with the murder of boxer John Abbey, Scotland Yard said.
Scotland Yard made two other arrests this morning. A 36-year-old man was detained in a "pre-planned" operation and is in custody at a south London police station. Another man was also arrested but no further information is available as yet, a Metropolitan police spokesman said.
John Nii Kacsu Abbey died at a bus stop in Croydon, south London, from a single stab wound to the heart on Thursday evening.
The 26-year-old boxer from Croydon - who was known to his friends as King - was on his way to visit his wife who had given birth earlier the same day.
The Ghanaian man and his young daughter were just a few hundred yards away from the Mayday hospital when the attack happened.
Officers said Mr Abbey became involved in an argument with a second man, believed to be a former colleague, before the fatal blow was struck.
They are still hunting two teenagers wearing hooded tops who fled the scene and may have been involved in the scuffle.
Mr Abbey's daughter was left watching in horror as paramedics tried to save her father's life.
Emergency services were called to busy London Road, west Croydon, shortly after 6.15pm on Thursday night.
The victim was taken to hospital where he was pronounced dead less than an hour later.
Detective Chief Inspector Cliff Lyons, who is leading the investigation, said the circumstances had left him and other officers extremely shocked.
He said the tragedy must "prick people's consciences" and encourage them to come forward with any information they have.
Mr Lyons said: "It is a double tragedy for the three-year-old, his wife and the son he will never see."
Police reiterated appeals for witnesses to come forward. Anyone with information is asked to call the incident room number on 020 8721 4961 or Crimestoppers on 0800 555 111.