Unseeded and unsung before the event began, Lee and Trotman beat the second seeds, Alejandro Hernandez, of Mexico and Mariano Puerta, of Argentina, 7-6, 6-4 after fighting back from 1-5 down in the opening set.
It was a magnificent comeback, with the British boys breaking first Hernandez and then Puerta to pull back to 5-5 before winning the tie-breaker 7-2.
Then one break, in the seventh game of the second set, was enough to enable 17-year-old Lee and 16-year-old Trotman to become the first British pair to win this event, after 80 minutes play.
Both are coached by the Australian Ian Barclay - who guided Pat Cash to his Wimbledon trimuph in 1987 - at the LTA School at Bisham Abbey, and for Trotman it was particularly pleasing.
The Ipswich boy was out of the game for much of last year with stress fractures in both feet and this year has been suffering from glandular fever.