Mauricio Pochettino is expected to make a number of changes for a tough clash in hostile Belgrade.
Danny Rose, who was subjected to racist abuse when he last played at the stadium for England Under-21s, will not play, with Ben Davies likely to come in.
Another former Swansea player Michel Vorm could make his debut, while Roberto Soldado has returned from injury and could start.
Mauricio Pochettino has used a secret weapon to help in his preparation for this match, with Serbian teenager Milos Veljkovic offering the manager some information.
"We used his information to our benefit," Pochettino explained. "Milos is a young and very good player and maybe he will have the chance to play.It is normal that he has information about Belgrade from his team-mates in the national team, he knows about the club and history. We use it - not too much - but we use it."
This is one of three long trips Tottenham will have to take to southern Europe, with Greek side Asteras and Turkish club Besiktas also in Group C.
Partizan enter the game in tremendous form, with five wins in their five league matches so far this season.
KICK-OFF: Thursday, 6pm
PAST THREE MEETINGS…
The two clubs have never played each other
Tottenham have lost just one of their last eight European away games – 1-0 at FC Dnipro Dnipropetrovsk in last season's round of 32 – with that run including five wins.
Partizan are unbeaten in six qualifying games this season (W4 D2) – though they lost out to Liverpool opposition Ludogorets Razgrad after two draws in the UEFA Champions League third qualifying round.
Partizan have lost their last three games against English opponents and have also lost their last three home fixtures against English teams since beating Newcastle United FC 1-0 in a 1998/99 UEFA Cup Winners' Cup tie.
Partizan to win: 4.5
Tottenham to win: 1.85
Live on ITV4 at 6pm.
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