Last year's Blue Square Bet Premier winners Fleetwood will welcome two-time European champions Nottingham Forest to Highbury for their first ever Capital One Cup tie.
Micky Mellon's side, who smashed the 100-point barrier on their way to the title, host Forest in the most eye-catching contest of round one which will begin the week commencing August 13.
Wolves start life under manager Stale Solbakken by welcoming Aldershot, who earned a money-spinning home tie with Manchester United in this competition last term, to Molineux.
Beaten finalists Cardiff travel to Northampton while Barnsley boss Keith Hill will go back to Rochdale for the first time since leaving them for Oakwell.
In other stand-out ties, York, who were promoted to npower League Two via the play-offs, face a trip to Doncaster, Paolo Di Canio's Swindon visit Gus Poyet's Brighton while Birmingham, who were winners of the competition two years ago, host Barnet at St Andrew's.
Derby County v Scunthorpe United
Rochdale v Barnsley
Port Vale v Burnley
Notts County v Bradford City
Hull City v Rotherham United
Fleetwood Town v Nottingham Forest
Carlisle United v Accrington Stanley
Doncaster Rovers v York City
Crewe Alexandra v Hartlepool United
Bury v Middlesbrough
Chesterfield v Tranmere Rovers
Sheffield United v Burton Albion
Blackpool v Morecambe
Leeds United v Shrewsbury Town
Preston North End v Huddersfield Town
Oldham Athletic v Sheffield Wednesday
Cheltenham Town v Milton Keynes Dons
Ipswich Town v Bristol Rovers
Stevenage v AFC Wimbledon
Exeter City v Crystal Palace
Yeovil Town v Colchester United
Birmingham City v Barnet
Bristol City v Gillingham
Northampton Town v Cardiff City
Plymouth Argyle v Portsmouth
Wolverhampton Wanderers v Aldershot Town
Walsall v Brentford
Millwall v Crawley Town
Torquay United v Leicester City
Dagenham & Redbridge v Coventry City
Peterborough United v Southend United
Swindon Town v Brighton & Hove Albion
Watford v Wycombe Wanderers
Charlton Athletic v Leyton Orient
Oxford United v AFC Bournemouth
Ties to be played week commencing 13th August 2012, however, if mutually agreed, The League will consider requests to play on Saturday 11th or Sunday 12th August 2012.