Spain boss Vicente del Bosque is set to wield the axe ahead of the do-or-die clash against Chile on Wednesday.
Barcelona duo Gerard Pique and Xavi may lose their places in the starting line-up but Del Bosque is expected to keep faith with goalkeeper Iker Casillas despite his horror show in the 5-1 defeat by Holland.
Spain know only a victory against their Group B opponents at the Maracana in Rio de Janeiro will suffice and Del Bosque said the team "are really hurting".
He said: "We still have a chance to progress in the World Cup but this situation has made a response from the players necessary if we want to advance."
Barcelona midfielder Andres Iniesta said the days since the Holland defeat had been agony, and pointed out that Spain had lost their opening match of the last World Cup - 1-0 to Switzerland - but still went on to win the tournament.
SPAIN: Group B team profile
SPAIN: Group B team profile
1/5 How they qualified
Those who were expecting Spain's qualifying campaign to be a formality were in for a surprise, as Vicente Del Bosque’s side struggled to secure qualification, only doing so with a 2-1 win over Belarus in the final game. Drawn in a group that contained France, it was far from a comfortable campaign for the world champions, despite going unbeaten in their eight games. They finished three points ahead of Les Bleus and topped the group as a result, but they did score fewer goals, with just 14 in total. Pedro’s tally of four in eight games epitomised the lack of firepower in the side as the Barcelona man finished top scorer for La Roja, with Manchester City striker Alvaro Negredo hitting three goals in just four games.
One of sharpest minds and most respected coaches in the game, former Real Madrid coach Vicente del Bosque has transformed the face of Spanish football. Granted, he has had some fantastic players to work with, but leading the nation to the 2010 World Cup and Euro 2012 is a special feat. He took over from Luis Aragones, who had lost just four of his 54 games in charge, and had just led Spain to their first European Championship title at Euro 2008. But Del Bosque was clearly not fazed by the task, becoming Spain’s most successful coach in their history, with his winning percentage just over 80 per cent. The 63-year-old has encouraged an attractive, attacking style of football which has seen Spain play some of the best passing football ever seen, and while the ‘tiki-taka’ style may have been started by Aragones, Del Bosque has prolonged its effectiveness.
3/5 Star man
Barcelona star Andres Iniesta is regarded as one of the world's top attacking midfielders, and has been instrumental to the dominance of Barcelona and Spain. While goals seem to come in clusters, with Iniesta never managing double figures for his club in a season, his assist tally continues to rise. World Cup Final win over the Netherlands three years ago, and remains a pivotal player under Del Bosque.
4/5 Emerging talent
Koke burst into European football’s collective consciousness last season in Atletico Madrid’s incredible La Liga winning season, which also saw Madrid’s second club reach the Champions league final. Koke, 22, is the youngest member of Spain’s 23-man squad, but his composed head in the centre of midfield belies his years. Koke has been linked with a move to Manchester United, Chelsea and Barcelona, but Los Rojiblancos chief Enrique Cerezo insists he is not for sale.
5/5 How they will line up (4-1-4-1)
Casillas, Azpilicueta, Pique, Ramos, Alba, Busquets, Fabregas, Iniesta, Xavi, Pedro, Costa
He said: "I wished we had played one or two days later because the waiting has been really hard.
"We must forget what has happened and put it behind us. We have two matches that are now like finals. We had a similar situation at the last World Cup and will give it our very best to win and get to second phase.
"Chile is a difficult team. We have to defend, fight one versus one and hope our creative players can make the difference."
Meanwhile, Spain's third-choice keeper David de Gea, the Manchester United player, is still injured, according to Spanish team officials.
Chile coach Jorge Sampaoli said he would make a decision close to kick-off on Juventus midfielder Arturo Vidal, who only played for the first half of the 3-1 win over Australia.
Vidal had knee surgery month ago and is still getting back to full fitness.
Sampaoli told a news conference: "We analyse his situation every single day. We know he's recovering now, he feels much better and he's here because he's really dedicated and willing to play."
Sampaoli said Spain will be fighting for their lives.
He added: "I think they will be difficult to beat. Don't forget they're one of the best teams in the world.
"I'm sure the game will be quite different because Spain are really strong and I'm pretty sure the Spain coach will have some sort of strategy."