Netherlands striker Robin van Persie is in the starting XI to face Spain in the teams' Group B match which kicks off at 8pm.
LIVE: The latest news on Day 2, including Cameroon v Mexico, Spain v Netherlands and Chile v Australia
The Manchester United striker had been in a doubt in the run-up to the game but Louis van Gaal is confident enough he is ready, selecting the former Arsenal player to lead the line against the reigning champions.
Arjen Robben is also in the side and will be among those eager to make amends for the 2010 final defeat they suffered. Also in the side from that night is Nigel de Jong, who memorably put in a high challenge on Xabi Alonso during the fractious match in South Africa.
Spain also have good news on the striker front with Diego Costa in the starting line-up.
The Brazilian-born Atletico Madrid striker has recovered from a right thigh muscle.
David Silva is also among those to start.
Spain: Iker Casillas, Cesar Azpilicueta, Jordi Alba, Gerard Pique, Sergio Ramos, Sergio Busquets, Xabi Alonso, David Silva, Xavi Hernandez, Andres Iniesta, Diego Costa.
Netherlands: Jasper Cillessen, Daryl Janmaat, Daley Blind, Bruno Martins Indi, Ron Vlaar, Stefan de Vrij, Nigel de Jong, Jonathan de Guzman, Wesley Sneijder, Arjen Robben, Robin van Persie.