The BBC received 172 viewer complaints after Mark Lawrenson made a sexist comment during Argentina's win against Switzerland on Tuesday.
The former Liverpool and Republic of Ireland defender said that Swiss striker Josip Drmic "should have put a skirt on" after his weak chipped effort when one-on-one went straight into the hands of Argentina goalkeeper Sergio Romero.
The BBC said: "We acknowledge that the remark by commentator Mark Lawrenson about Switzerland's Josip Drmic was inappropriate and we apologise for any offence caused by it."
Broadcasting watchdog OfCom also received six complaints, but has not yet decide whether to formally investigate.
The BBC have come in for some strong criticism during the tournament, with co-commentator Phil Neville widely ridiculed for his commentary during England's defeat against Italy and perceived bias as Brazil beat Chile via a penalty shoot-out.
mark lawrenson "should've put a skirt on" after player failed with a chip shot. Pathetic sexist statement from a tasteless man @BBCSport— Chris Howard (@ChrisGore7) July 1, 2014
Half-time report: Embarrassing, sexist commentary from Mark Lawrenson, suggesting that misfiring striker should wear a skirt. @BBCSport— Paul Smith (@paulsmithmusic) July 1, 2014
SHOULD HAVE PUT A SKIRT ON!! Don't appreciate that casual sexist remark by Mark Lawrenson. Not one bit #bbcworldcup— Megan Enlli (@Cau_Dy_Geg) July 1, 2014
@BBCSport please remove Mark Lawrenson from all future BBC Sport showings. His constant derogatory and now sexist remarks have gone too far!— David Taylor (@Big_Hamish) July 1, 2014
What a sexist comment by Mark Lawrenson. Not impressed!!!! #bbcworldcup— Brenda (@brenXmj88n92) July 1, 2014
Just a slightly sexist comment by Mark Lawrenson there— Llwyd™ (@Llwyd11) July 1, 2014
Did anyone else just notice Mark Lawrenson's horribly sexist remark?— Alex Sambrook (@sambroooook) July 1, 2014
"Should put a skirt on" lmao Mark Lawrenson I really hope you lose your job you Neanderthal— Hannah ✌️ (@hannahscraic) July 1, 2014
Neville's commentary prompted 400 viewer complaints.
ITV had problems on the opening night of the tournament, when ITV Player crashed because of the high volume of people trying to watch the World Cup opener.