A group of four football fans, said to be Chelsea supporters, are being investigated by the Police following claims they were making racist chants.
The Daily Mail report that the four fans were taken off a Euston to Manchester service at 10.30pm, following Chelsea's Capital One Cup final win over Tottenham.
Transport police were called to the service at Stoke following complaints from other passengers. A witness is quoted as saying the fans were being "nasty and offensive" before police got on the train and "marched them off".
A spokesman from British Transport Police said: "Shortly after 10.30pm on Sunday, 1 March, officers were called to meet a Euston-to-Manchester Piccadilly train at Stoke, following reports of racist and abusive behaviour by a number of passengers, said to be football fans.
"Colleagues from Staffordshire Police also attended. Officers asked four men to leave the train at Stoke. We have spoken to two witnesses, and investigations continue.
"Rail passengers and staff have the right to travel and work in peace. Abusive behaviour on trains and at stations is unacceptable, and we will not hesitate to investigate any such incidents."
BTP are examining mobile phone footage of the alleged incident on the train from London to Manchester which the force obtained from a witness.
A Chelsea spokesman later said: "We are aware of the reports but it is not clear at this time if this incident involves Chelsea supporters.
"If it did then we will assist the British Transport Police with their enquiries."
The allegations comes in the wake of incidents on the Paris Metro, where a black passenger was not allowed to board the train, and St Pancras, where seven men are alleged to have been chanting racist songs.
Supporters were warned prior to the Wembley contest to be on their best behaviour and were greeted by a sign which read 'Racist chanting may lead to arrest' on Wembley Way.
Speaking publicly for the first time about the Paris incident on February 20, Mourinho was asked how offensive it was that the supporters used the name of club captain John Terry to justify their actions. A Chelsea spokesman intervened by saying: "We're not interested in any excuses or stories to try and cover these actions."
Terry was given a four-match ban and fined £220,000 in 2012 after being found guilty by the Football Association of racially abusing QPR defender Anton Ferdinand. In Terry's case, the regulatory commission stated that the Chelsea captain was "not a racist".
The Chelsea skipper scored the opening goal in Sunday's success and was named man of the match.
Additional reporting from PA
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