Everton defender Phil Jagielka is to have surgery on a badly gashed ankle, manager David Moyes has confirmed.
Moyes said he did not know how long Jagielka would be out but the 30-year-old must now be doubtful for next week's FA Cup quarter-final against Wigan.
The incident also reminded Moyes of some of the treatment goalkeeper Tim Howard received in the two previous cup matches against Oldham.
Howard was unable to face Reading after suffering a knock in the fifth-round replay win over over the League One side on Tuesday and was replaced by back-up Jan Mucha.
"I'm not happy with the challenges on the goalkeeper in the last two games that have put him out and I am not happy with the challenge today that puts Phil Jagielka out," said the manager.
"I'm not one who minds challenges. I like a bit of that, if that's what it is.
"I don't mind robust challenges but not one that puts out arguably one of my best players."
Howard's absence ended the American's run of 210 consecutive Premier League appearances.
Moyes said: "I thought Jan did very well but it came about because the goalkeeper wasn't protected in the first game against Oldham and they felt it was right they could do that again in the second game.
"In the end the goalkeeper has been injured because of decisions which should have been deemed free-kicks long before."