Welford Road, the home of Leicester Tigers and the biggest club rugby ground in England, has been axed as a potential venue for the 2015 World Cup.
The ground, which has been Leicester's home for 120 years, has been left off the long list of 17 stadia published by tournament organisers England Rugby 2015.
Gloucester's Kingsholm is the only Aviva Premiership rugby ground on a list that includes Twickenham, the Olympic Stadium, Wembley, the Millennium Stadium and some of the biggest club football stadia in the country.
The home grounds of Manchester United, Newcastle, Sunderland, Aston Villa and Southampton have all been included after expressing an interest in staging World Cup matches.
Outside of the Premier League, ER2015 have included the Amex Stadium in Brighton, Bristol City's Ashton Gate, Pride Park in Derby, Coventry's Ricoh Arena, Elland Road in Leeds and Stadium MK in Milton Keynes.
Leicester is still represented by the King Power Stadium, which has a capacity of over 32,000 and, tournament organisers believe, more suitable off-field facilities than Welford Road.
Critics argue the World Cup should be utilising more rugby grounds but ER2015 have to sell 2.9million tickets in order to cover the £80million guarantee owed to the International Rugby Board.
ER2015 will confirm the final list of 10-12 venues early in the new year, once the tournament match schedule has been formulated. The pool draw is being held on December 3 in London.
Arsenal's Emirates Stadium was in the original bid document but it does not feature on the shortlist, replaced by the Olympic Stadium.
ER2015 are in talks with the Olympic Park Legacy Company over the future use of the stadium, which has obvious attractions following the success of London 2012.