The actor has previously played the dapper MI6 spy in Casino Royale, Quantum of Solace, Skyfall and Spectre, and is set to star in one last 007 feature, No Time to Die.
The sketch will see Tate revive her character of Nan from Noughties sketch hit The Catherine Tate Show.
In the scene, the foul-mouthed curmudgeon finds herself cleaning the office of the head of the Secret Intelligence Service, where she soon comes face-to-face with Bond himself.
Airing on BBC One this Friday (19 March), the Comic Relief special will take the form of a three-hour variety show hosted by Sir Lenny Henry.
Davina McCall, Paddy McGuinness, Alesha Dixon and David Tennant will also feature in the broadcast, as well as a host of guest stars.
Another of the evening’s highlights is a crossover between Fleabag and Normal People, while Keira Knightley also appears in a skit about “the greatest disaster movie never made”.
No Time to Die is currently scheduled to be released in cinemas on 30 September, having been delayed multiple times due to the pandemic.
Red Nose Day will air on Friday 19 March at 7pm on BBC One.
Join our new commenting forum
Join thought-provoking conversations, follow other Independent readers and see their replies