Clarkson announced on 31 July that the farm team was "devastated" after a pregnant cow contracted the disease, with tests for the beloved bull Endgame proving inconclusive.
Following failed biannual tests for some cattle, the farm is now under restrictions, preventing the buying or selling of cows, though the Diddly Squat shop remains unaffected.
The infected pregnant cow, carrying twins, will be culled, a decision Clarkson described as "awful" and emotionally distressing for the farm.
Bovine TB is a chronic respiratory disease that primarily affects cattle, often leading to culling to control its spread, though human transmission is very rare.