JDM had a weigh-in scare on Friday, initially registering 170.4lb and facing the prospect of losing his title on the scales, but he successfully made the 170lb limit at 169.8lb after removing his shorts.
This bout marks Makhachev's first fight at 170lb, having previously relinquished his lightweight belt after a record four successful defences.
Makhachev opted to move up a division after JDM defeated his training partner, Belal Muhammad, for the welterweight title in May.
Both fighters enter Saturday's main event on significant winning streaks, with JDM having won 18 consecutive bouts and Makhachev 15 straight fights.