Protesters have been refused permission to appeal against their eviction from the Occupy London camp outside St Paul's Cathedral.
The group challenged the High Court's decision that the City of London Corporation's move to evict its camp was "lawful and justified".
The Court of Appeal's decision to refuse the application means the corporation is free to clear the site.
"All appeals denied but fight not over," Occupy London said on Twitter. "Having a central focus point at St Paul's can still be maintained [without] residential tents but this isn't a setback."
The City of London Corporation called on the protesters to leave immediately. Occupy London, which campaigns against corporate greed, set up the camp on 15 October. There are about 120 tents at the site. Two large tents which housed the "kitchen" and "university" have already been removed by the protesters as they were donated and they did not want them destroyed.