Aileen McGlynn relinquished her Paralympic tandem one-kilometre time-trial title and had to settle for silver alongside pilot Helen Scott at the London 2012 velodrome - but the British duo are already eyeing success in Rio de Janeiro.
McGlynn, who is partially sighted, and Scott clocked one minute 9.469 seconds to finish 0.550secs behind Australia's Felicity Johnson and Stephanie Morton, who stole victory in a Paralympic record of 1min 8.919secs.
It was the first time McGlynn has tasted Paralympic defeat in the event after the 39-year-old from Glasgow won gold in Athens and Beijing with Ellen Hunter as pilot.
But McGlynn and Scott, 22 from Halesowen, are determined to chase glory in Brazil in four years' time after finishing second to the Australian pair at the last two World Championships and now in London.
"This was our main focus," said a disappointed McGlynn. "We came into it really well prepared and we thought we had it. It's bike racing, it doesn't always go the way you wanted to.
"I've definitely got more to offer. We've got more to come, it just didn't work out today.
"It all depends on what team management think, whether I'm getting past it, or I go on to Rio."
Scott was a team-mate of Olympic rider Jess Varnish before turning her attentions to tandem racing in April 2010 and although she has the potential to switch to the Olympic disciplines, her goal remains Paralympic glory.
Scott, wearing a necklace featuring a tandem bike, said: "I love riding the tandem, so I've got no plans to go over. I still want a gold medal with Aileen.
"That's our mission and we're not going to stop until we get it.
"We came for gold, but to get a medal in your home Games, and for me being my first Games, I'm over the moon.
"We put our hearts and souls into the kilo over the last six months after the world champs, producing personal best times every time we stepped on the track.
"It wasn't fast enough to win it, but it was closer than it was at the world champs."
McGlynn added: "She's a big part of the Paralympic squad now and I'm sure she'll want to continue to Rio."
New Zealand's Phillipa Gray and Laura Thompson claimed bronze in 1:11.245, with Lora Turnham and Fiona Duncan fourth in 1:11.479. The British pair's main event is Sunday's 3km tandem pursuit.
Turnham said: "We've never gone faster, that's all we can do.
"I love competing - it's what I'm here to do. I'm just here to enjoy myself."
Duncan said: "To come out here and do a personal best, we're pleased."
Ireland's Catherine Walsh and pilot Francine Meehan were fifth in 1:12.864.
Walsh said: "It's a personal best and Irish record. We're delighted. We're happy. This was just a dress rehearsal for the 3km for us."
Hosts Britain were poised for a strong afternoon session, with Jody Cundy and Jon-Allan Butterworth in action in the C4/5 one-kilometre and Mark Colbourne riding for gold in the men's C1 3km individual pursuit after qualifying fastest in a world record of 3:53.970.
Colbourne finished second to Li Zhang Yu in the C1-2-3 one-kilometre time-trial on day one and watched the Chinese rider set a world record of 4:00.235 in 3km pursuit qualifying before improving the mark himself.
The 42-year-old Welshman was this afternoon set to meet Li in the race for gold.
Colbourne said: "I've been training on a sub-four-minute schedule and I think today the crowd gave me that extra couple of miles per hour."