The Jewish Children's Museum in Brooklyn has been evacuated due to a bomb threat, the NYPD said.
The threat was sent to the museum around 9.30am and police are investigating.
It comes two days after a series of bomb threats prompted evacuations at several Jewish community centres across the US.
Every US senator has signed a letter calling for greater federal help in tackling the new wave of bomb threats.
“We’ve never seen such a period of concentrated threats against the Jewish community,” New York Mayor Bill de Blasio told a news conference.
“The last few weeks are more troubling than anything I’ve seen in many, many years.”