Jim Gannon has parted company with Port Vale after just three months and 15 games in charge at Vale Park.
Mark Grew has been appointed as caretaker manager and will take charge of first-team affairs, starting with the visit of Hereford on Tuesday night.
Vale slipped out of the npower League Two play-off places following Saturday's 3-0 defeat at Accrington, prompting angry Vale fans to call for Gannon's head.
The former Stockport and Peterborough manager led the team to four wins, four draws and seven defeats during his short tenure, despite the club occupying a top-seven position for the majority of the season.
It marks the end of a tumultuous time at Vale Park for Gannon, who only took over in January following Micky Adams' defection to Sheffield United.
Gannon and assistant Geoff Horsfield were involved in an argument on the way to Aldershot on February 25, which led to both men leaving the team coach. The bust-up was dealt with internally.
He then refused to speak to the media after the defeat at Accrington and was led inside the Crown Ground for his own safety while fans protested against him.
There was also a scene at the end of the game where defender Exodus Geohaghon and Valiants fans had a confrontation as the players left the pitch.