Addressing the crowd, the US talk show personality told the crowd he was worried because Trump, a fervent tweeter, hadn't posted any messages in a while.
Taking matters into his own hands, Kimmel sent him: 'Hey @realDonaldTrump, u up?'
Not content with just that, the host pushed the joke even further by sending a follow-up which read: '#Merylsayshi,' a message referencing the President's criticism of Meryl Streep after the actor took aim at the Republican at the Golden Globes in January.
The tweet has received over 164,000 retweets after a mere 15 minutes making it the most popular Oscars moment since Ellen DeGeneres' celebrity selfie in 2015.
Kimmel took aim at Trump in a politically charged opening monologue which, among other things, saw him directly brand the President a 'racist.'
Join our new commenting forum
Join thought-provoking conversations, follow other Independent readers and see their replies