The prominent physicist, who had a bout of pneumonia last year, said "it would not be wise for me to have a bucket of cold water pour over me… but my children Robert, Lucy and Tim gallantly volunteered to take the challenge for me."
He then urged everyone to donate to the MNDA to "eliminate this terrible disease".
In the family’s driveway, his offspring took on the challenge before Hawking, 72, nominated the Director of the Science Musem, Ian Blatchford and the Chancellor and Vice-Chancellor of Cambridge University - Lord Sainsbury and Professor Sir Leszek Borysiewicz.
MNDA is the British equivalent of the ALS Association in the US, which has so far received $95million in donations since the viral and organic ice bucket challenge took off at the end of the last month.
Hawking was diagnosed with motor neurone disease at the age of 21 and had been given just two years to live.
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