Osvaldo Carvalho, from Hanwell, died after he was attacked near Ealing Common on Tuesday afternoon.
He was pronounced dead at the scene, despite the efforts of paramedic teams to save him.
Kamaljit Sohal, Sukhminder Sohal and Michael Sohal have now been charged with his murder, London's Metropolitan Police said in a statement.
The trio will appear in custody at Ealing Magistrates’ Court later this week.
Police said Carvalho and two of his friends where involved in a confrontation with another group .
Carvalho ran away and the group chased and stabbed him.
"A young man has tragically lost his life and another family has been left grieving," Detective Chief Inspector Noel McHugh said after the murder. The death of this man will no doubt impact all those who knew him, his family, friends and also the wider community in the area where this attack took place.”
He added that there was nothing to suggest that the attack was linked to two other murders in west London in the hours before the incident.
Mr Carvalho’s death came hours after a man was murdered at nearby Hillingdon station and less than two days after another 22-year-old was stabbed to death in Southall.
There have been more than 110 homicides so far in London this year amid ongoing fears about knife crime in the capital.
Last year, the number of killings in London rose to the highest level for a decade with 132 homicides reported over the year.
Anyone with information on the Ealing stabbing is asked to call the Incident Room number on 020 8785 8099 or 101 quoting Operation Reedley.
Agencies contributed to this report