Mr Yousaf condemned reports from the United Nations that 1.1 million Palestinians were ordered to vacate northern Gaza within 24 hours, by the Israeli military.
The region subject to the order includes Gaza’s biggest hospital, which Human Rights Watch has said is dealing with more than 6,000 casualties, and two major refugee camps.
The UN described the military order as “devastating”.
Mr Yousaf’s in-laws are trapped in Gaza, and his wife Nadia El-Nakla has spoken of her torment at the prospect of her relatives living in a refugee camp with no food or water.
Mr Yousaf said there was “no justification for the death of innocent men, women and children”.
Posting on Twitter, formerly known as X, Mr Yousaf said: “UN has said the order to move 1.1m people in 24 hours will lead to ‘devastating humanitarian consequences’.
“The international community must step up and demand an end to collective punishment.
“Enough. There can be no justification for the death of innocent men, women & children.”