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Labour has refused to support a Commons motion that would give the public a fresh referendum on Brexit , dealing a heavy blow to the chances of the amendment passing.
The party leadership is instead expected to order its MPs to abstain during the vote, which will take place when MPs debate a potential delay to Brexit tonight.
The motion, tabled by The Independent Group's Sarah Wollaston, demands that Theresa May delay Brexit “for the purposes of legislating for and conducting a public vote in which the people of the United Kingdom may give their consent" for either leaving the EU on the terms of a deal agreed by Parliament or remaining in the bloc.
It will be the first time that MPs have voted on whether to call another public vote and comes on another day of high drama in Westminster.
Ms Wollaston's motion is backed MPs from Labour, the SNP, Plaid Cymru, the Liberal Democrats and The Independent Group.
But the Labour leadership said it had come at the wrong time. The party has said it supports a public vote to prevent "a damaging Tory Brexit" or a no-deal outcome, and did not rule out backing a motion on another referendum at a later date.
Speaking in the Commons, Sir Keir Starmer, the shadow Brexit secretary said: "Today is about the question of whether Article 50 should be extended and whether we can find a purpose.
"Many colleagues in and out of this place absolutely supportive of the cause of a People's Vote vehemently disagree with this amendment being tabled and voted on today."
He added: "Those pressing this amendment seem to be out of step with the vast majority of co-campaigners who are campaigning for exactly the same cause.
"They may genuinely have a difference of opinion but we will not be supporting [amendment] H tonight."
A historic moment: thousands march to demand Final Say on BrexitShow all 65 1 /65A historic moment: thousands march to demand Final Say on Brexit A historic moment: thousands march to demand Final Say on Brexit An estimated 700,000 people marched through London to demand a final say on the withdrawal agreement
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit Red smoke from a canister hangs in the air as around 100,000 demonstrators march through London during a People's Vote anti-brexit demonstration savings banners and placards Anti-Brexit People's Vote March for the Future in London
Rex
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit Mayor of London Sadiq Khan takes part in the People's Vote March for the Future in London, a march and rally in support of a second EU referendum.
PA
A historic moment: thousands march to demand Final Say on Brexit Final Say campaigners take part in the peoples vote march for the future in London 20/10/2018 Protesters wearing final Say shirts and holding placards
Angela Christofilou
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit MP Chuka Umunna (left) and MP Vince Cable (right) as MP Anna Soubry (centre) addresses Anti-Brexit campaigners at a rally after the People's Vote March for the Future in London, a march and rally in support of a second EU referendum.
PA
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit A People’s Vote march attendee calls for a Final Say
Angela Christofilou
A historic moment: thousands march to demand Final Say on Brexit Demonstrators with banners 'We're with EU' during the People's Vote March for the Future in London, Britain, 20 October 2018. Reports state that the 'March for the Future' is to be led by a column of young people and call for a People’s Vote on the Brexit deal. After marching through central London, there will be a rally on stage in Parliament Square, including speeches from Mayor of London Sadiq Khan.
EPA
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit Protesters at London’s march for the future in October
The Independent
A historic moment: thousands march to demand Final Say on Brexit Editor of The Independent Christian Broughton speaks to demonstrators in Parliament Sqaure after they take part in a march calling for a People's Vote on the final Brexit deal, in central London on October 20, 2018. - Britons dreading life outside Europe gathered from all corners of the UK to London on Saturday to try to stop their country's looming breakup with the EU.
AFP/Getty
A historic moment: thousands march to demand Final Say on Brexit A poster at the March for the Future in October
The Independent
A historic moment: thousands march to demand Final Say on Brexit Anti-Brexit campaigners take part in the People's Vote March for the Future in London, a march and rally in support of a second EU referendum.
PA
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit Final Say campaigners take part in the peoples vote march for the future in London 20/10/2018
Angela Christofilou
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit Anti-Brexit campaigners take part in the People's Vote March for the Future in London, a march and rally in support of a second EU referendum.
PA
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit Demonstrators hold placards as they take part in a march calling for a People's Vote on the final Brexit deal, in central London on October 20, 2018. - Britons dreading life outside Europe gathered from all corners of the UK to London on Saturday to try to stop their country's looming breakup with the EU.
AFP/Getty
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou
A historic moment: thousands march to demand Final Say on Brexit Final Say campaigners take part in the peoples vote march for the future in London 20/10/2018 campaigner wrapped in EU flag
Angela Christofilou
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit
Angela Christofilou/The Independent
A historic moment: thousands march to demand Final Say on Brexit Final Say campaigners take part in the peoples vote march for the future in London 20/10/2018
Angela Christofilou
A historic moment: thousands march to demand Final Say on Brexit Demonstrators pass Trafalgar Square as they take part in a march calling for a People's Vote on the final Brexit deal, in central London on October 20, 2018. - Britons dreading life outside Europe gathered from all corners of the UK to London on Saturday to try to stop their country's looming breakup with the EU.
AFP/Getty
A historic moment: thousands march to demand Final Say on Brexit Demonstrators wave Union and European flags and hold up placards as they pass Trafalgar Square, taking part in a march calling for a People's Vote on the final Brexit deal, in central London on October 20, 2018. - Britons dreading life outside Europe gathered from all corners of the UK to London on Saturday to try to stop their country's looming breakup with the EU.
AFP/Getty
A historic moment: thousands march to demand Final Say on Brexit Demonstrators hold placards as they take part in a march calling for a People's Vote on the final Brexit deal, in central London on October 20, 2018. - Britons dreading life outside Europe gathered from all corners of the UK to London on Saturday to try to stop their country's looming breakup with the EU.
AFP/Getty
A historic moment: thousands march to demand Final Say on Brexit A demonstrator holds a message during a march calling for a People's Vote on the final Brexit deal, in central London on October 20, 2018. - Britons dreading life outside Europe gathered from all corners of the UK to London on Saturday to try to stop their country's looming breakup with the EU.
AFP/Getty
A historic moment: thousands march to demand Final Say on Brexit Anti-Brexit campaigners take part in the People's Vote March for the Future in London, a march and rally in support of a second EU referendum.
PA
A historic moment: thousands march to demand Final Say on Brexit Demonstrators take part in the 'People's Vote March for the Future,' in central London, Britain, 20 October 2018. Reports state that the 'March for the Future' is to be led by a column of young people and call for a People’s Vote on the Brexit deal. After marching through central London, there will be a rally on stage in Parliament Square, including speeches from Mayor of London Sadiq Khan
EPA
A historic moment: thousands march to demand Final Say on Brexit A protester brandishes an Independent t-shirt during the Brexit March
Angela Christofilou
A historic moment: thousands march to demand Final Say on Brexit Anti-Brexit campaigners take part in the People's Vote March for the Future in London, a march and rally in support of a second EU referendum
PA
A historic moment: thousands march to demand Final Say on Brexit Tens of thousands of people take part in People's Vote March for the Future in central London. The march organised by the People's Vote campaign is led by young people calling for a People's Vote on the Brexit deal
Rex
A historic moment: thousands march to demand Final Say on Brexit Anti-Brexit campaigners take part in the People's Vote March for the Future in London, a march and rally in support of a second EU referendum
PA
His announcement was met with cries of "shame on you" from The Independent Group MPs.
But Sir Keir said the People's Vote campaign had also said it was not the right moment to force a Commons vote on another referendum.
A spokesperson for the campaign said: “The People’s Vote campaign does not instruct its supporters in parliament on how to vote. We recognise there is a range of opinions on when to press the case for the public being given the final say , which means some of these MPs will vote for the Wollaston amendment, some may vote against, and some will abstain.
“But we do not think today is the right time to test the will of the House on the case for a new public vote. Instead, this is the time for parliament to declare it wants an extension of Article 50 so that, after two-and-a-half years of vexed negotiations, our political leaders can finally decide on what Brexit means."
MPs cheer when Theresa May asks if they want another Brexit referendum The Best for Britain campaign, which is also campaign for another referendum, took a similar position. A spokesperson said: "We believe bringing this issue before parliament today will not truly test the will of the House for a public vote.
"The debate today must focus on the pressing need for an extension to Article 50 to confirm parliament’s rejection of a no deal cliff-edge on 29 MNarch and protect the country from a damaging disorderly exit."
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