Ms Gates, 56, filed for divorce from Mr Gates, 65, on Monday, stating that the marriage had become "irretrievably broken”.
According to the filing, Ms Gates will not request a change of name and asked for the couple’s belongings and property to be divided by the terms of a separation agreement.
In a statement, the couple said the divorce came “after a great deal of thought and a lot of work on our relationship”.
It continued: “Over the last 27 years, we have raised three incredible children and built a foundation that works all over the world to enable all people to lead healthy, productive lives”.
“We continue to share a belief in that mission and will continue to work together at the foundation, but we no longer believe we can grow together as a couple”.
Famous for philanthropic work around the world, the couple married in 1994 before the Bill & Melinda Gates Foundation began in 2000.
It is the biggest philanthropic organisation in the US and has given more than $50 billon (£36 billon) over the past two decades towards fighting poverty and disease.
The couple first met at Microsoft — the billion dollar technology firm — and have three adult children, Phoebe, 18, Rory, 21, and Jennifer, 25.
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