The 28-year-old started the 1-1 draw with Eibar on the bench before coming on in the second half but limped off the pitch during stoppage time as Barcelona finished the game with 10 men having made all their substitutions.
The Brazilian has now undergone an operation on the injury to his left knee ending his campaign.
“Philippe Coutinho underwent surgery successfully by Dr Rodrigo Lasmar on a meniscal cyst in his left knee under the supervision of the club’s medical services,” a statement read.
“The player is not available for selection and his recovery will dictate his return to action.”
The Brazilian has played only 14 games this season in all competitions scoring three goals.
Barcelona are second in the league with 65 points, trailing Atletico Madrid by a point.
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