With the sides drawing 2-2 and just seconds of added time remaining, the ball fell to Kane in the middle of the pitch and he tried his luck at beating former Arsenal player Wojciech Szczesny from range.
The gamble paid off and Kane curled the ball over the helpless Juventus goalkeeper to seal the win.
"It's probably one of the best goals in my career," Kane said. "I saw the 'keeper was quite far off his line a few times, so I thought I would take the chance if I had the opportunity. I saw him off his line and fortunately it went in."
Watch the goal below:
I think it was a great goal," said Pochettino. "Unbelievable from Harry. I think it is good for him to start the pre-season scoring. I think to beat Juventus of course that wasn't the priority to win the game but to compete in our best way and in the end to win is always better than not winning.
"We are happy with the performance but the most important thing is that we are happy that all the players finished in a good condition to train tomorrow and build step by step our fitness.!
A youthful Spurs side took the lead in the first half when Erik Lamela converted Gianluigi’s parry in the six-yard box after some good work from Son Heung-min.
Juventus battled back after the restart and took the lead through Cristiano Ronaldo after Gonzalo Higuain struck.
£55m signing Tanguy Ndombele made an instant impact to assist Lucas Moura with his first touch as the game looked like it was heading for a draw, but Kane’s moment of brilliance separated the sides in Singapore.
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