The 28-year-old Republic of Ireland full-back has signed a four-year deal with Spurs, having made over 300 appearances during his time at Molineux.
"We are delighted to announce the signing of Matt Doherty from Wolverhampton Wanderers," Tottenham confirmed on their official website.
Having come through the youth system at League of Ireland side Bohemians, Doherty moved to Wolves in 2010 and went on to establish himself in the side.
He played in every Premier League game of the 2018/19 season and helped Nuno Espirito Santo's men to back-to-back seventh-place finishes and a Europa League quarter-final.
He joins Spurs after Wolves accepted a bid understood to be £15m and becomes Jose Mourinho's third signing of the summer following the arrivals of Pierre-Emile Hojbjerg and Joe Hart.
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