The Three Lions will travel to Leeds to face Costa Rica on Thursday before flying out to Russia for the World Cup.
Harry Kane, Raheem Sterling, Jordan Henderson, Jamie Vardy and Dele Alli were all involved in the session on Wednesday morning.
Stand-by players Tom Heaton, Jake Livermore and Adam Lallana also took part.
Fellow stand-by man Lewis Cook is currently with the England Under-21s for the Toulon Tournament with the Young Lions facing Scotland in the semi-finals on Wednesday.
England face Tunisia on June 18 before playing Panama on June 24 and Belgium four days later in Group G.
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