The 143mph blast was the fastest serve of any sort during the tournament so far and also the second-fastest second serve in ATP history.
Kyrgios was raging after losing a challenge on his first serve and then smashed down an unstoppable, untouchable second.
Only Ivo Karlovic's 144mph effort at the 2007 Legg Mason Classic has topped it, according to ATP statistics.
Kyrgios went to the opposite extreme moments later with a gentle under-arm ace.
Nadal won the first set 6-3 but Kyrgios took the second 6-3.
The match took place in a fiery atmosphere on Centre Court with a war or words between the two beforehand spilling over on to the court with Kyrgios also enraged with the umpiring earning a code violation in the process.
Additional reporting by Reuters
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