The First Minister indicated that rather than being settled by the courts, the matter should be decided by politicians.
She told BBC Scotland: “It is absolutely essential that if the will of the Scottish Parliament is for a referendum then that should be respected.
“I don't think there is any need, nor is there any intention, to see a matter that should be settled politically end up in the courts.”
There had been speculation the Scottish Government could be about to mount a legal challenge after the First Minister pointed out the decision to reserve constitutional matters for Westminster to deal with had never been tested in the courts.
In a question-and-answer session following a speech at Stanford University in California, Ms Sturgeon also said it was “quite a vague term”.
Scottish Liberal Democrat leader Willie Rennie demanded answers, calling on the First Minister to provide “clarity on exactly what you are planning to do”.
The Scottish Government insists a second referendum is needed to give Scots a choice between Brexit and independence.
Ms Sturgeon has proposed such a vote be held between autumn 2018 and spring 2019, when the terms of the UK's exit deal from the European Union are known.
In pictures: European parliament Brexit discussions
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While the decision to leave the EU was backed by a majority of voters in the 2016 referendum, 62% of Scots who took part opted for remain.
The SNP leader is engaged in a stand-off with Theresa May over the prospect of a fresh ballot on independence, with the Prime Minister saying “now is not the time” for such a vote to be held.
Press Association
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