Sancho replaces Bukayo Saka in England's attack, while Kieran Trippier drops out of the starting line-up to make way for the returning Mason Mount.
Trippier’s omission sees England switch from the back five which worked so well in Tuesday’s 2-0 win against Germany, despite Ukraine playing the same system as Joachim Low’s side.
Jack Grealish is named among the substitutes despite his match-winning turn at Wembley. Ben White and Reece James are left out of the squad, along with Saka who is suffering from a knock.
Ukraine are missing Atalanta winger Ruslan Malinovskiy, who is a surprise omission from Andriy Shevchenko’s squad. Oleksandr Zinchenko and Andriy Yarmolenko both start, as does their joint top-scorer Roman Yaremchuk.
England: Pickford; Walker, Stones, Maguire, Shaw; Phillips, Rice, Mount; Sancho, Kane, Sterling.
Substitutes: Ramsdale, Johnstone, Grealish, Henderson, Rashford, Trippier, Mings, Coady, Calvert-Lewin, Foden, Chilwell, Bellingham.
Ukraine: Bushchan; Karavaev, Zabarnyi, Kryvtsov, Matviyenko, Mykolenko; Sydorchuk, Zinchenko, Shaparenko; Yarmolenko, Yaremchuk.
Substitutes: Sobol, Sudakov, Stepanenko, Marlos, Pyatov, Makarenko, Tsygankov, Bezus, Zubkov, Trubin, Tymchyk, Dovbyk.
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