The England captain scored deep into injury-time to spark wild celebrations among his teammates, who could scarcely believe their eyes after Kane hit a first-time effort from halfway over the head of Juventus goalkeeper Wojciech Szczesny.
Spurs had initially take the lead thanks to Erik Lamela's first-half effort, despite manager Mauricio Pochettino fielding an experimental team against Maurizio Sarri's full-strength Juventus side. But the Italian side's talent soon told in the second half as Gonzalo Higuain came off the bench to equalise before Cristiano Ronaldo put them ahead inside four minutes of each other.
Lucas Moura levelled soon after for the Premier League side with Kane introduced for the second half, while Sarri handed first appearances to Juventus new boys Matthijs de Ligt and Adrien Rabiot. But the best was saved for last as Kane produced his moment of magic to seal the victory to keep Tottenham's pre-season plans on track. Re-live the action below.
What time does it start?
Juventus vs Tottenham kicks off at 12:30 BST.
Where can I watch it?
The International Champions Cup clash will be shown live on Premier Sports 1 from 12:00pm.
Juventus: Buffon; De Sciglio, Rugani, Bonucci, Cancelo; Matuidi, Can, Bernardeschi; Pjanic, Mandzukic, Ronaldo.
Subs: Szczesny, Pinsoglio, Loria, de Ligt, Demiral, Di Pardo, Coccolo, Beruatto, Rabiot, Kastanos, Muratore, Toure', Frederiksen, Pereira, Higuain, Mavididi.
Tottenham: Gazzaniga, Walker-Peters, Alderweireld, Tanganga, Georgiou, Winks, Skipp, Lamela, Dele, Son, Parrott.
Welcome to The Independent's live coverage of the International Champions Cup clash between juventus and Tottenham, brought to you from the Singapore National Stadium.
Tottenham supporters will have to wait a little longer for Ndombele to make his first start in a Spurs shirt.
Spurs XI: Gazzaniga, Walker-Peters, Alderweireld, Tanganga, Georgiou, Winks, Skipp, Lamela, Dele, Son, Parrott.
Eriksen is on the bench while Lloris misses out due to tonsillitis.
Walker-Peters vs Ronaldo should be fun...
Juventus XI: Buffon, Cancelo, Rugani, Bonucci, De Sciglio, Emre Can, Pjanic, Matuidi, Bernardeschi, Mandzukic, Ronaldo
De Ligt is named on the bench in Maurizio Sarri's first game in charge, while Ramsey is still working towards full fitness. Rabiot and Higuian are also players that could feature as substitues.
Pochettino places a huge amount of faith in the young players in his squad and there are some huge tests for some of the teenagers out there.
17-year-old Troy Parrott comes up against Leonardo Bonucci, a veteran regarded as one of the best Italian defenders of a generation.
The entire defence will be tested by the power of Ronaldo, including Tanganga, 20, and Georgiou, 22.
Even aged 34, there has been no sign of Ronaldo slowing down in recent years. He never gives less than everything he's got in training, and today we have the first chance to see what this season may hold for him.
Not long to go, kick-off in Singapore is just under 10 minutes away.
So, we are likely to get our first glimpse of new Tottenham signing Tanguy Ndombele.
Here's why he might be the most important Spurs signing in, well, ever...
We're off in Singapore!
After a coin toss, that was in fact more of a card toss...don't ask, Tottenham begin the match.
Spurs begin their International Champions Cup campaign against Juventus. These really are the moments that you look back on as a player at the end of your career and think: 'I was there'...
1: Strong start from Spurs, straight back to Tanganga and hoofed out for a throw in Juventus' half. Welcome to pre-season, everybody!
What a man
4: It appears that Mandzukic is playing through the middle and Ronaldo is playing with what seems to be a free role, either that or he's ignoring everything Sarri said to him ahead of the game.
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